The main objective of this project is an in-depth characterization of patients with severe community-acquired pneumonia. This approach of “deep phenotyping” goes far beyond existing observational studies (CAPNETZ, PROGRESS) and aims at providing new data and ideas for multiscale modeling, disease simulation and experimental theory validation, producing new insights into the understanding of processes involved in CAP-associated lung injury and alveolo-capillary barrier failure, and identifying new multilevel patterns in patients with severe CAP. As an expansion of the PROGRESS study, ventilated pneumonia patients will be subjected to a detailed clinical and extensive biochemical characterization. Moreover, transcriptomics, proteomics and metabolomics will be applied. In particular, we will explore the alveolar microbial and host proteome, as well as the respiratory microbiome.

Deep phenotyping in patients with severe CAP and new analyses in CAP
Patients for “deep phenotyping” will be recruited in selected PROGRESS study centers as an add-on to the current PROGRESS study. Recruitment and characterization of eligible patients is expected to be very demanding, requiring special motivation and support of study centers. We aim for enrolling n=100 mechanically ventilated CAP-patients. Beyond peripheral blood measurements, we will characterize and analyse the respiratory compartment in established cohorts.